期刊
JOURNAL OF EXPERIMENTAL BIOLOGY
卷 208, 期 17, 页码 3263-3273出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jeb.01739
关键词
mosquito; carbonic anhydrase; histochemistry; midgut pH; carbonic anhydrase inhibitor
类别
资金
- FIC NIH HHS [TW1130] Funding Source: Medline
- NIAID NIH HHS [R01 AI 45098, AI45430] Funding Source: Medline
- NIGMS NIH HHS [GM 25154] Funding Source: Medline
We have previously demonstrated the involvement of carbonic anhydrase (CA) in larval mosquito midgut physiology. In this study, we used Hansson's histochemistry to examine the distribution of the enzyme in the midgut of Aedes aegypti, Aedes albopictus, Culex quinquefasciatus, Culex nigripalpus, Ochlerotatus taeniorhynchus, Anopheles albimanus and Anopheles quadrimaculatus adult mosquitoes. Additionally, we quantitated CA content in the anterior and posterior midgut of adult males and females from these species using the 180 isotope exchange method coupled to mass spectrometry. We also tested the effect of CA inhibitors such as methazolamide and acetazolamide in the alkalization of the midgut in females from these species. Our results indicate that CA is present in the midgut of adults from the species studied and that it appears to be preferentially associated with the posterior midgut in both males and females. CA inhibitors appear to have a profound effect on midgut pH indicating that this enzyme might play a key role in the maintenance of this pH.
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