期刊
BIOLOGICAL & PHARMACEUTICAL BULLETIN
卷 28, 期 9, 页码 1736-1742出版社
PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/bpb.28.1736
关键词
Radix Bupleuri; saikosaponin; conversion; orthogonal design; HPLC
In this study, the quantitative analysis of saikosaponins from Radix Bupleuri in China was performed by high performance liquid chromatography. Saikosaponin-a and -d were converted completely into saikosaponin b(1) and -b(2) by mild acid treatment. Distinctive measuring of these converted diene-saponins provided a rapid and selective method for the determination of saikosaponin-a and A in commercial samples of Radix Bupleuri. The conditions of extraction and conversion of saikosaponins were optimized using orthogonal design L-9(3(4)). The HPLC analysis was performed on ODS-C-18 column with a flow rate of 1.0 ml/min and detection wavelength of 250 nm. Well resolved chromatograms of saikosaponin-b(1) and -b(2) were obtained with an isocratic elution of acetonitrile : 1% formic acid water (37.5 : 62.5). Calibration curves of saikosaponin-b(1) and -b(2) were linear in the range of 4.9-98.0 mu g/ml and 3.5-71.0 mu g/ml, respectively. The average recovery of saikosaponin-b(1) and -b(2) were 98.3% (RSD=3.1%) and 96.4% (RSD=1.8%), respectively. Seventeen samples of different species and habitats of Radix Bupleuri were analyzed by the developed HPLC method.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据