期刊
MICROBIOLOGY-SGM
卷 151, 期 -, 页码 2987-2999出版社
MICROBIOLOGY SOC
DOI: 10.1099/mic.0.28040-0
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- NIAID NIH HHS [R01 AI 47442] Funding Source: Medline
Candida albicans produces and accumulates large amounts of the polyols D-arabitol and glycerol in culture, and/or in infected mammalian tissues. However, the effects of environmental stresses on production and accumulation of these polyols, and the means by which polyol production and accumulation are regulated have not been studied. C. albicans grown in glucose at 30 degrees C (i) produced maximal amounts of glycerol within 6 h, (ii) produced maximal amounts of D-arabitol and ribitol within 12 h, and (iii) released most of these polyols into the extracellular environment. C. albicans responded to osmotic and citric acid stress by producing and accumulating more glycerol, and to temperature and oxidative stresses by producing more D-arabitol. The increase in intracellular glycerol was proportional to extracellular osmolarity, suggesting that glycerol functions as an osmolyte. The MAP kinase Hog 1p is required for wild-type glycerol production in several fungal species subjected to osmotic stress, but it is not known if Hog 1 p plays a role in regulating D-arabitol production. Therefore, two C. albicans hog 1 null mutants were constructed and tested for the ability to produce glycerol and D-arabitol in response to environmental stresses. The ability to grow and produce glycerol when exposed to osmotic or citric acid stresses, and to produce D-arabitol when exposed to oxidative stress, was partially dependent on Hog 1 p, but the ability to produce D-arabitol when exposed to temperature stress was Hog 1 p independent. These results imply that multiple pathways regulate glycerol and D-arabitol synthesis in C. albicans.
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