期刊
GENERAL AND COMPARATIVE ENDOCRINOLOGY
卷 143, 期 2, 页码 151-160出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygcen.2005.03.007
关键词
mosquitofish; 17 beta-trenbolone; masculinization; gonopodium; ovotestis androgen receptor alpha; androgen receptor beta
Endocrine disrupting chemicals can affect normal hormone dependent processes through numerous mechanisms, including ligand mimicky. 17 beta-Trenbolone (TB), a pharmaceutical, androgenic, anabolic steroid, is a potent agonist of androgen receptors, and has been extensively used as a growth promoter for beef cattle in the US. The effects of TB on adult and newborn mosquitofish (Gambusia affinis affinis) were examined. Two forms of mosquitofish androgen receptor (AR), AR alpha and AR beta, were cloned. The mRNA expression levels of AR alpha and AR beta were transiently increased in the anal fin of adult females at day 3 following exposure to TB (1-10 mu g/L) or methyltestosterone (MT) (0.1-10 mu g/L), a pharmaceutical androgen used as a positive control. Gonopodium differentiation from the adult female anal fin was induced after 28 days of exposure to TB (1-10 mu g/L) or MT (0.1-10 mu g/L). Gonopodium differentiation also was induced in all mosquitofish fry exposed for 28 days to 0.3, 1 or 10 mu g/L TB. Furthermore, spermatozoa were observed histologically in the testes of male fry exposed for 28 days to I or 10 mu g/L TB; spermatozoa are normally observed only in the testes of mature males. Surprisingly, all female fry exposed for 28 days to I or 10 mu g/L TB displayed the formation of an ovotestis, as spermatozoa were found in the ovary. Thus, TB, like MT, induced masculinization of the anal fin accompanied by a transient up-regulation of AR alpha and AR beta in adult females. TB also induced differentiation of the anal fin into a gonopodium in fry of both sexes, stimulated precocious spermatogenesis in the testes of males and the formation of ovotestes in females. (c) 2005 Elsevier Inc. All rights reserved.
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