期刊
DIGESTIVE DISEASES AND SCIENCES
卷 50, 期 9, 页码 1661-1668出版社
SPRINGER
DOI: 10.1007/s10620-005-2912-2
关键词
enteric neurons; neuronal plasticity; vasoactive intestinal peptide; nitric oxide; alpha-ketoglutarate; lipopolysaccharide
Enteric bacteria execute, via lipopolysaccharide (LPS), a pathogenic role in intestinal inflammation. The effects of LPS on survival and neurotransmitter expression in cultured porcine myenteric neurons were investigated. Myenteric neurons were isolated and cultured for 6 days in medium, in LPS (100 ng/ml) with or without alpha-ketoglutarate or the nitric oxide synthase (NOS) inhibitor L-NAME, in alpha-ketoglutarate or in the NO donor SNAP. Neuronal survival and expression of vasoactive intestinal peptide (VIP) and NOS were evaluated by immunocytochemistry. Addition of LPS significantly decreased neuronal survival; only 40% survived, compared to controls run in parallel. The LPS-induced neurotoxic effect was not counteracted by the simultaneous presence of alpha-ketoglutarate or L-NAME. Either SNAP or alpha-ketoglutarate influenced neuronal survival. Culturing, particularly in the presence of LPS, markedly increased the proportion of VIP-immunoreactive neurons; NOS-immunoreactive neurons were unchanged. The reported LPS-induced neurotoxicity indicates loss of enteric neurons as a consequence of intestinal inflammation.
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