Cytotoxicity on V79 and HL60 Cell Lines by Thiolated-β-Cyclodextrin-Au/Violacein Nanoparticles

In vitro cytotoxicity of a supramolecular system composed by violacein complexed by,beta-cyclodextrin-thiol-protected gold nanoparticles (violacein @ beta-CD-S(CH2)(6)-S-Au) on V79 and HL60 cell lines is described. The gold nanoparticles were prepared and modified in a single step involving reduction of tetrachloroaurate ions with sodium borohydride in the presence of thiol derivatized beta-cyclodextrin. Inclusion complexation of violacein into cyclodextrin cavities occurred by mixing an aqueous solution of the gold nanoparticles with an acetone solution of violacein, being evidenced by UV/visible absorption spectroscopy According to cell viability measurements based on the MTT assay (3-(4,5-dimethylthiazole-2-yl)-2,5-biphenyl tetrazolium bromide) the supramolecular system was found to maintain the cytotoxic effects compared to free violacein on HL60 cells and in addition is less cytotoxic to normal (V79) cells.