期刊
FISH PATHOLOGY
卷 40, 期 3, 页码 137-138出版社
JAPAN SOC FISH PATHOL DEPT FISHERIES-FAC AGR
DOI: 10.3147/jsfp.40.137
关键词
koi herpesvirus; KHV; virucidal effect; disinfectant; ultraviolet
The virucidal effects of ultraviolet (UV) irradiation, heat treatment and disinfectants against koi herpesvirus (KHV) were evaluated using KF-1 cells. KHV (KHV-I strain) was completely inactivated by UV irradiation at a dose of 4.0 x 103 mu Ws/CM2 or heating to temperatures above 50 degrees C for 1 min. KHV was completely inactivated by 200 mg/L iodophor, 60 mg/L benzalkonium chloride solution or 30% ethyl alcohol for 20 min. When chlorine concentration was measured after mixing with virus supernatant containing medium and fetal bovine serum, 97.5% and 98.5% reduction in infectivity was observed at 0.30 mg/L of chlorine for 30 s and 20 min, respectively. In practice, a tenfold concentration of chlorine (i.e. 3 mg/L) will be recommended to inactivate KHV completely.
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