Virucidal effects of ultraviolet, heat treatment and disinfectants against koi herpesvirus (KHV)

The virucidal effects of ultraviolet (UV) irradiation, heat treatment and disinfectants against koi herpesvirus (KHV) were evaluated using KF-1 cells. KHV (KHV-I strain) was completely inactivated by UV irradiation at a dose of 4.0 x 103 mu Ws/CM2 or heating to temperatures above 50 degrees C for 1 min. KHV was completely inactivated by 200 mg/L iodophor, 60 mg/L benzalkonium chloride solution or 30% ethyl alcohol for 20 min. When chlorine concentration was measured after mixing with virus supernatant containing medium and fetal bovine serum, 97.5% and 98.5% reduction in infectivity was observed at 0.30 mg/L of chlorine for 30 s and 20 min, respectively. In practice, a tenfold concentration of chlorine (i.e. 3 mg/L) will be recommended to inactivate KHV completely.