期刊
JOURNAL OF IMMUNOLOGY
卷 175, 期 5, 页码 3117-3122出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.175.5.3117
关键词
-
类别
资金
- NIAID NIH HHS [AI46653, 2T32AI0075-33, T32AI0726-19, AI50073, AI42767] Funding Source: Medline
The precise mechanisms that govern the commitment of CD4 T cells to become Th1 or Th2 cells in vivo are incompletely understood. Recent experiments demonstrate colocalization of the IFN-gamma R chains with the TCR during activation of naive CD4 T cells, suggesting that association of these molecules may be involved in determining lineage commitment. To test the role of IFN-gamma and its receptor in the generation of Thl Ag-specific CD4 T cells, we analyzed mice after infection with Listeria monocytogenes or lymphocytic choriomeningitis virus. In the absence of IFN-gamma, Ag-specific CD4 T cells were generated in response to both these infections. In addition, IFN-gamma-producing (Th1) Ag-specific CD4 T cells were generated in mice lacking the ligand-binding chain of the IFN-gamma R (IFN-gamma RI-/-) or the signaling chain (IFN-gamma R2(-/-)). There was no increase in the number of IL-4-producing Ag-specific CD4 T cells, nor was there a decrease in the expression of T-bet in the absence of functional IFN-gamma signaling, indicating that the cells were committed Thl cells. Thus, both chains of the IFN-gamma are dispensable for the generation of Thl Ag-specific CD4 T cells after infection in vivo.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据