期刊
FREE RADICAL BIOLOGY AND MEDICINE
卷 39, 期 5, 页码 567-583出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2005.04.016
关键词
SOD; mitochondria; iron; oxidative stress; apoptosis; nitrooxide; free radical
资金
- NHLBI NIH HHS [HL07305-01, 5P01HL68769-01] Funding Source: Medline
The purpose of this study was to test the hypothesis whether Mito-carboxy proxyl (Mito-CP), a mitochondria-targeted nitroxide, inhibits peroxide-induced oxidative stress and apoptosis in bovine aortic endothelial cells (BAEC). Glucose/glucose oxidase (Glu/GO)-induced oxidative stress was monitored by dichlorodihydrofluorescein oxidation catalyzed by intracellular H2O2 and transferrin receptor-mediated iron transported into cells. Pretreatment of BAECs with Mito-CP significantly diminished H2O2- and lipid peroxide-induced intracellular formation of dichlorofluorescene and protein oxidation. Electron paramagnetic resonance (EPR) studies confirmed the selective accumulation of Mito-CP into the mitochondria. Mito-CP inhibited the cytochrome c release and caspase-3 activation in cells treated with peroxides. Mito-CP inhibited both H2O2- and lipid peroxide- induced inactivation of complex I and aconitase, overexpression of transferrin receptor (TfR), and mitochondrial uptake of Fe-55, while restoring the mitochondrial membrane potential and proteasomal activity. In contrast, the untargeted carboxy proxyl (CP) nitroxide probe did not protect the cells from peroxide-induced oxidative stress and apoptosis. However, both CP and Mito-CP inhibited superoxide-induced cytochrome c reduction to the same extent in a xanthine/xanthine oxidase system. We conclude that selective uptake of Mito-CP into the mitochondria is responsible for inhibiting peroxide-mediated Tf-Fe uptake and apoptosis and restoration of the proteasomal function. (c) 2005 Elsevier Inc. All rights reserved.
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