期刊
MOLECULAR ECOLOGY NOTES
卷 5, 期 3, 页码 546-548出版社
WILEY
DOI: 10.1111/j.1471-8286.2005.00983.x
关键词
barley scald; genetic variation; microsatellites; population structure; Rhynchosporium secalis
Fifteen primer pairs were designed for 14 polymorphic microsatellite loci, which were isolated and characterized from genomic libraries of Rhynchosporium secalis. Conditions for multiplexing and simultaneous genotyping of up to eight loci in a single run are described. The number of alleles per polymorphic locus ranged from two to 13 in populations from Switzerland and Australia. Genotypic diversity ranged from 61.5 to 66.7. Gene diversity ranged from 0.08 to 0.89 for individual polymorphic loci, with averages of 0.54 and 0.62 for the populations from Switzerland and Australia, respectively. Variable levels of polymorphism make these ideal markers for population genetic analyses.
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