期刊
VISION RESEARCH
卷 45, 期 20, 页码 2659-2667出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.visres.2005.03.020
关键词
retina; bipolar cell; GABA receptors; chloride transporters
Gamma-amino butyric acid (GABA) has been characterized as inhibitory neurotransmitter through chloride mediated channels in the adult nervous system. However, using gramicidin perforated patch-clamp recordings from rod bipolar cells dissociated from retinas of adult mice, we find that GABA is capable of inducing cell depolarization. Currents mediated by GABA(A) and GABA(C) receptors were further isolated by the use of GABA receptor specific blockers. In rod bipolar cells dissociated from the mouse retina, activation of GABAA receptors located at the cell dendrites induces ionic currents which show a reversal potential of -33 mV. However, local activation of GABA(C) receptors located at the axon terminal induces ionic currents with a reversal potential of -60 mV. According to Nernst equation, the dendrites of rod bipolar cells of the mouse retina would have a high intracellular chloride concentration ([Cl-](i)) and there must be an intracellular gradient in [Cl-](i), being the [Cl-](i) more elevated in the dendrites than in the axon terminal. The depolarizing effect of GABA at the dendrites of rod bipolar cells may contribute to the lateral interaction in the mammalian retina, thereby enhancing visual discrimination of stimuli input. (c) 2005 Elsevier Ltd. All rights reserved.
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