期刊
FEMS MICROBIOLOGY LETTERS
卷 250, 期 1, 页码 33-38出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.femsle.2005.06.057
关键词
soil contamination; Nitrosospira sp; genetic diversity; real-time PCR
类别
Chemolithotrophic ammonia-oxidising bacteria (AOB) present in oil-contaminated landfarming soil were studied over two growing seasons in 1999 and 2000. The number of AOB (4-9 x 10(5)) cells g(-1) of dry soil) determined with the quantitative polymerase chain reaction (real-time PCR) and the rate of potential ammonium oxidation (0.05-0.28 mu g NO2--N g(-1) of dry soil h(-1)) indicated the presence of stable AOB populations. Denaturing gradient gel electrophoresis (DGGE) profiling and sequence analysis of PCR-amplified AOB 16S rRNA genes showed dominance of Nitrosospira-like sequences in clusters 2 and 3. The present results from the chronically oil-contaminated landfarming soil support the suggested importance of Nitrosospira-like AOB in terrestrial environments. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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