期刊
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
卷 193, 期 3, 页码 849-856出版社
MOSBY-ELSEVIER
DOI: 10.1016/j.ajog.2005.01.029
关键词
hypoxic-ischemia encephalopathy; neonatal rat; neural stem cell; differentiation
Objective: We examined whether progenitor neural stem cells can differentiate successfully into mature neurons and astrocytes in a rat model of neonatal hypoxic-ischemic encephalopathy. Study design: Seven-day-old Wistar Rats were subjected to hypoxic-ischemic stress. At days to 7 Neonatal rat after hypoxic-ischemic stress, 5-bromodeoxyuridine (an early marker of cell proliferation) Was Neural stem cell injected, and the brains were retrieved at 14, 28, and 42 days after hypoxic-ischemic stress. Immunohistochemical and immunofluorescent studies were carried out for 5-bromodeoxyuridine, neuronal nuclear antigen (a marker protein of matured neuron), and glial fibrillary acidic protein (a protein marker of mature astrocytes). Results: Only 1% of neuronal nuclear antigen-positive and 4.6% of glial fibrillary acidic protein-positive cells could be detected among the 5-bromodeoxyuridine-immunopositive cells in the p infarcted area of the cortex and the striatum, respectively, at 14 days after hypoxic-ischemic stress. There were no such double-staining cells at 28 and 42 days after hypoxic-ischemic stress. Conclusion: The intrinsic ability for neurologic self-repair was limited at the maturation step after hypoxic-ischemic stress in the neonatal rat brain. (c) 2005 Mosby, Inc. All rights reserved.
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