期刊
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
卷 289, 期 3, 页码 F585-F592出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajprenal.00050.2005
关键词
cyclooxygenases; extracellular nucleotides; arginine vasopressin; purinergic; aquaporin; inner medullary collecting duct; prostaglandin E-2
资金
- NIDDK NIH HHS [DK-61183] Funding Source: Medline
Circulating vasopressin levels change in hydrated and dehydrated conditions and thus control osmotic water permeability (P-f) of the inner medullary collecting duct (IMCD). Prostaglandin E-2 (PGE(2)) antagonizes vasopressin-induced Pf of IMCD. Previously, we showed that activation of P2Y2 receptor (P2Y2-R) in IMCD results in production and release of PGE(2), and P2Y2-R mRNA and protein are significantly elevated in inner medullas of hydrated rats compared with dehydrated rats. Therefore, we examined whether the altered expression of P2Y2-R in hydrated and dehydrated states is associated with corresponding changes in P2Y2R-mediated PGE2 release by the IMCD. Rats were hydrated by providing sucrose water as the sole drinking fluid or dehydrated by water deprivation for 2 days. This resulted in high output-low osmolality and low output-high osmolality urines in hydrated and dehydrated rats, respectively. In hydrated rats, there was a significant increase in tubular fluid PGE(2), measured indirectly by assessing the urinary PGE(2) metabolite. Stimulation of freshly isolated IMCD preparations in vitro with P2Y2-R agonist (ATP gamma S) showed a marked increase in the release of PGE(2) in hydrated rats compared with normal rats. These responses were blunted in the IMCD prepared from dehydrated rats. The P2Y2-R-mediated PGE(2) release in the IMCD of hydrated rats was mediated largely by cyclooxygenase (COX)-1 as COX-1-specific inhibitor valeroyl salicylate completely blocked the release. The COX-2-specific inhibitor N5398 had only a modest and insignificant inhibitory effect. In conclusion, the increased sensitivity of purinergic-prostanoid interaction seen in the IMCD of hydrated rats may represent a novel vasopressin-independent regulatory mechanism of IMCD function.
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