期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 280, 期 35, 页码 30723-30734出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M500435200
关键词
-
Schlafen- 1 ( Slfn- 1), the prototypic member of the family of proteins, was described as an inducer growth arrest in T- lymphocytes and causes a cell cycle in NIH3T3 fibroblasts prior to the G(1)/ S transition. Slfn- 1 exerts its effects on the cell cycle is not currently known. We report that synchronized murine fibroblasts expressing Slfn- 1 do not exit G(1) when stimulated fetal calf serum, platelet- derived growth factor BB PDGF- BB) or epidermal growth factor ( EGF). The induction of cyclin D1 by these stimuli was blocked in the presence of Slfn- 1 as were all downstream cell cycle processes. of cyclin D1 in growth- arrested, Slfn- 1cells induced an increase in cell growth consistent with this protein being the biological target of 1. Activation of the mitogen- activated protein kinase by EGF or phorbol 12- myristate 13- acetate was by Slfn- 1 expression. PDGF signaling was, almost completely blocked. This was due to a of PDGF receptor expression in Slfn- 1- expressing consistent with Slfn- 1 blocking the cell cycle in G1 PDGF receptor expression is normally down- regulated. Finally, overexpression of Slfn- 1 inhibited the activation of the cyclin D1 promoter. Slfn- 1 therefore causes a cycle arrest during G1 by inhibiting induction of cyclin D1 by mitogens.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据