期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1086, 期 1-2, 页码 2-11出版社
ELSEVIER
DOI: 10.1016/j.chroma.2005.05.093
关键词
gas chromatography; comprehensive two-dimensional; time-of-flight mass spectrometry; lipids
Most lipids are a complex mixture of classes of compounds such as fatty acids, fatty alcohols, diols, sterols and hydroxy acids. In this study, the suitability of comprehensive two-dimensional gas chromatography coupled to a time-of-light mass spectrometer is studied for lipid characterization in complex samples. With lanolin, a refined wool wax, as test sample, it is demonstrated that combined methylation plus silylation is the preferred derivatization procedure to achieve (i) high-quality GC x GC separation and (ii) easily recognizable ordered structures in lipid analysis. Optimization of the GC x GC column combination, the influence of the temperature. programme on the quality of the separation, and the potential and limitations of automated TOF-MS-based identification are discussed. The combined power of a 2D separation, ordered structures and MS detection is illustrated by the identification of several minor sample constituents. (c) 2005 Elsevier B.V. All rights reserved.
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