期刊
JOURNAL OF IMMUNOLOGY
卷 175, 期 6, 页码 3508-3515出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.175.6.3508
关键词
-
类别
资金
- NCI NIH HHS [CA82430, CA90571] Funding Source: Medline
- NIAID NIH HHS [AI21256] Funding Source: Medline
- NIDDK NIH HHS [DK43726] Funding Source: Medline
Helios, a member of the Maros family of DNA-binding proteins, is expressed in multipotential lymphoid progenitors and throughout the T lineage. However, in most B lineage cells, Helios is not expressed, suggesting that its absence may be critical for B cell development and function. To test this possibility, transgenic mice were generated that express Helios under the control of an Ig mu enhancer. Commitment to the B cell lineage was unaltered in Helios transgenic mice, and numbers of surface IgM(+) B cells were normal in the bone marrow and spleen. However, both bone marrow and splenic B cells exhibited prolonged survival and enhanced proliferation. B cells in Helios transgenic mice were also. hyperresponsive to Ag stimulation. These alterations were observed even though the concentration of ectopic Helios in B lineage cells, like that of endogenous Helios in thymocytes, was well below the concentration of Ikaros. Further evidence that ectopic Helios expression contributes to B cell abnormalities was provided by the observation that Helios transgenic mice developed metastatic lymphoma as they aged. Taken together, these results demonstrate that silencing of Helios is critical for normal B cell function.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据