期刊
TALANTA
卷 67, 期 3, 页码 548-554出版社
ELSEVIER
DOI: 10.1016/j.talanta.2005.06.043
关键词
DNA recovery; amino-modified silica-coated magnetic nanoparticles; sever acute respiratory syndrome (SARS)
In this paper, an improved recovery method for target ssDNA using amino-modified silica-coated magnetic nanoparticles (ASMNPs) is reported. This method takes advantages of the amino-modified silica-coated magnetic nanoparticles prepared using water-in-oil microemulsion technique, which employs amino-modified silica as the shell and iron oxide as the core of the magnetic nanoparticles. The nanoparticles have a silica surface with amino groups and can be conjugated with any desired bio-molecules through many existing amino group chemistry. In this research, a linear DNA probe was immobilized onto nanoparticles through streptavidin conjugation using covalent bonds. A target ssDNA(I) (5 '-TMR-CGCATAGGGCCTCGTGATAC-3 ') has been successfully recovered from a crude sample under a magnet field through their special recognition and hybridization. A designed ssDNA fragment of severe acute respiratory syndrome (SARS) virus at a much lower concentration than the target ssDNA(I) was also recovered with high efficiency and good selectivity. (c) 2005 Elsevier B.V. All rights reserved.
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