期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1087, 期 1-2, 页码 38-44出版社
ELSEVIER
DOI: 10.1016/j.chroma.2005.04.029
关键词
peptide library; E. coli; high throughput screening; immobilised metal affinity chromatography; cryogels; green fluorescent protein
A method for high throughput screening of Green Fluorescent Proteins carrying metal binding tags in bacteria was developed. A random four amino acids tag-peptide library was successfully generated in E. coli. A 96-microtiter plate assembled with metal-iminodiacetic acid small cryogel columns was used for library screening. For the first time we were able to simultaneously screen a metal binding peptide tags library obtained from E. coli against different metal ions. From screening 25 different tags, three clones were able to bind to all metal ions studied (Ni2+, Zn2+, Co2+ and Cd2+). It was clearly demonstrated that the new construct could facilitate the screening of large peptide libraries. (c) 2005 Elsevier B.V. All rights reserved.
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