Long-term delivery of superoxide dismutase and catalase entrapped in poly(lactide-co-glycolide) microspheres: In vitro effects on isolated neonatal porcine pancreatic cell clusters

To counterbalance the restricted availability of pancreatic islet tissue for transplant in Type I Diabetes Mellitus (TIDM), new methods to provide viable and functional islet cells need to be established. We report on our approach to enhance in vitro viability and function of isolated neonatal pancreatic porcine cell clusters (NPCCs) by co-culturing them with PLGA microsphere entrapped, slowly release superoxide dismutase and catalase. These powerful antioxidizing agents were shown to significantly improve morphology, viability and function, as assessed by microscopy, molecular, biochemical and functional studies, of the incubated NPCCs, as compared to control. Preliminarily, in vitro exposure of isolated NPCCs to slow release microsphere-embedded SOD and CAT could permit or contribute to overcome hurdles associated with scarcity in islet tissue procurement for transplant in TIDM. (c) 2005 Elsevier B.V. All rights reserved.