期刊
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
卷 1745, 期 3, 页码 382-392出版社
ELSEVIER
DOI: 10.1016/j.bbamcr.2005.05.010
关键词
estrogen receptor alpha; estrogen receptor beta; mitochondrion; SaOS-2; HepG2
The localization of estrogen receptors alpha (ER alpha) and beta (ER beta) in osteosarcoma SaOS-2 and hepatocarcinoma HepG2 cells was studied by immunofluorescence labelling and confocal laser scanning microscopy, as well as by subcellular fractionation and immunoblotting of the proteins of the fractions with respective antibodies. In both cell types, ERa was localized mainly in the nucleus, particularly concentrated on nuclear structures, which on the basis of their staining with pyronin and with antibodies against the nucleoli-specific Ki67 antigen and C23-nucleolin, were characterized as nucleoli. A faint, diffuse ERa staining was also observed in the cytoplasm. ER beta was specifically enriched at the site of the mitochondria, visualized by labelling with the vital dye CMX and antibody against the mitochondrial-specific cytochrome oxidase subunit I. Immunoblotting experiments corroborated the immunofluorescence labelling distribution of ER alpha and ER beta. These findings support the concept of a direct action of steroid/thyroid hormones on mitochondrial functions by way of their cognate receptors and also suggest a direct involvement of ER alpha in nucleolar-related processes. (c) 2005 Elsevier B.V. All rights reserved.
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