期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
卷 1709, 期 3, 页码 214-219出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbabio.2005.08.003
关键词
Drosophila; mitochondria; superoxide; glycerol phosphate dehydrogenase; complex III
The topology of superoxide generation by sn-glycerol 3-phosphate dehydrogenase and complex III in intact Drosophila mitochondria was studied using aconitase inactivation to measure superoxide production in the matrix, and hydrogen peroxide formation in the presence of superoxide dismutase to measure superoxide production from both sides of the membrane. Aconitase inactivation was calibrated using the known rate of matrix superoxide production from complex I. Glycerol phosphate dehydrogenase generated superoxide about equally to each side of the membrane, whereas centre o of complex III in the presence of antimycin A generated superoxide about 30% on the cytosolic side and 70% on the matrix side. (c) 2005 Elsevier B.V. All rights reserved.
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