期刊
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
卷 111, 期 12, 页码 2473-2484出版社
SPRINGER
DOI: 10.1007/s10482-018-1136-3
关键词
Methanotrophs; India; Rice fields; Candidatus Methylocucumis oryzae'; Novel genus; Pale pink; Strain Sn10-6
类别
资金
- DBT BioCARe, India
- MACS Agharkar Research Institute, India
An elliptical to cucumber-shaped methanotroph with large cells was isolated from a rice rhizosphere in Western India. Strain Sn10-6 is one of the first methanotrophs to be isolated from Indian rice fields. Cells of Sn10-6 are Gram-negative, motile, large in size (3-6 mu mx1.5-2 mu m) and contain intracellular cytoplasmic membrane stacks. Colonies of Sn10-6 and liquid cultures have a pale pink colour. Strain Sn10-6 was initially isolated under micro-oxic conditions but later adapted to grow under fully oxic conditions. The major fatty acids present were identified as C(16:1)6c, C(16:1)7c and C-16:0 and ubiquinone was found to be the major quinone. The 16S rRNA gene sequence of strain Sn10-6 displays high similarity to the genes of Methylovulum psychrotolerans Sph1(T) (93.6%) followed by Methylosarcina fibrata AML-C10(T) (93.5%) and about 90-93% similarity to the genes of known species of Type I methanotrophic genera from the family Methylococcaceae. The draft genome information indicated that the G+C content of strain Sn10-6 is 43.9mol%. Phylogenetic trees using 16S rRNA gene and the particulate methane mono-oxygenase sequences unequivocally placed Sn10-6 close to the genus Methylovulum. Based on the 16S rRNA gene differences, morphological characters and draft genome information, strain Sn10-6 (=MCC 3492 =KCTC 15683) is described here as the type strain of a novel species within a new genus, Candidatus Methylocucumis oryzae'.
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