期刊
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
卷 106, 期 6, 页码 1147-1155出版社
SPRINGER
DOI: 10.1007/s10482-014-0284-3
关键词
Bacillus cihuensis; DNA-DNA relatedness; Phylogenetic analysis; Polyphasic taxonomy
类别
资金
- Agricultural Bioresources Institute, Fujian Academy of Agricultural Sciences, PR China
- international cooperation project of Chinese Ministry of Science and Technology [2012DFA31120]
- Natural Science Foundation of China (NSFC) [31370059]
- 948 project of Chinese Ministry of Agriculture [2011-G25]
- 973 program earlier research project [2011CB111607]
- project of agriculture science and technology achievement transformation [2010GB2C400220]
A Gram-positive, moderately halotolerant, rod-shaped, spore forming bacterium, designated strain FJAT-14515(T) was isolated from a soil sample in Cihu area, Taoyuan County, Taiwan. The strain grew at 10-35 A degrees C (optimum at 30 A degrees C), pH 5.7-9.0 (optimum at pH 7.0) and at salinities of 0-5 % (w/v) NaCl (optimum at 1 % w/v). The diagnostic diamino acid of the peptidoglycan of the isolated strain was meso-diaminopimelic acid and major respiratory isoprenoid quinone was MK-7. Major cellular fatty acids were anteiso-C-15:0 (40.6 %), iso-C-15:0 (20.7 %) and the DNA G+C content of strain FJAT-14515(T) was 37.1 mol %. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain FJAT-14515(T) belongs to the genus Bacillus, and was most closely related to the reference strains of Bacillus muralis DSM 16288(T) (97.6 %) and Bacillus simplex DSM 1321(T) (97.5 %). Levels of DNA-DNA relatedness between strain FJAT-14515(T) and the reference strains of B. muralis DSM 16288(T) and B. simplex DSM 1321(T) were 27.9 % +/- A 3.32 and 44.1 % +/- A 0.57, respectively. Therefore, on the basis of phenotypic, chemotaxonomic and genotypic properties, strain FJAT-14515(T) represents a novel species of the genus Bacillus, for which the name Bacillus cihuensis sp. nov. is proposed. The type strain is FJAT-14515(T) (=DSM 25969(T) = CGMCC 1.12697(T)).
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