Thioclava atlantica sp nov., isolated from deep sea sediment of the Atlantic Ocean

A taxonomic study was carried out on strain 13D2W-2(T), which was isolated from a sulphur-oxidizing bacterial consortium, enriched by the deep-sea sediment of the Atlantic Ocean. The isolate was observed to be Gram-negative, oxidase-and catalase-positive, short rodshaped and motile by means of a flagellum. Growth was observed at salinities from 0.5 to 12 % and at temperatures from 4 to 41 degrees C, and the strain found to be able to reduce nitrate but not degrade gelatin. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 13D2W-2(T) belongs to the genus Thioclava, with highest sequence similarity of 97.8 % to Thioclava dalianensis DLFJ1-1(T), followed by Thioclava pacifica TL 2(T) (97.7 %), while the sequence similarities to other members of the genus were all below 97.0 %. The digital DNA: DNA hybridization estimated values between strain 13D2W-2(T) and, respectively, T. dalianensis DLFJ1-1(T) and T. pacifica TL 2(T) were 22.6 +/- 2.4 and 25.6 +/- 2.4 %. The ANI values between strain 13D2W-2(T) and T. dalianensis DLFJ1-1 T and T. pacifica TL 2(T) were 78.49 and 81.91 % respectively. The principal fatty acid identified was Summed Feature 8 (C-18:1 omega 7c/omega 6c) (74.38 %). The isoprenoid quinone of strain 13D2W-2(T) was identified as Q10 (100 %). The major polar lipids of strain 13D2W-2(T) were found to be comprised of phosphatidylethanolamine, phosphatidylglycerol, an aminophospholipid, a glycolipid and three unknown phospholipids. The G+C content of the chromosomal DNA was determined to be 65.3 mol%. The combined genotypic and phenotypic data show that strain 13D2W-2(T) represents a novel species of the genus Thioclava, for which the name Thioclava atlantica sp. nov. is proposed, with the type strain13D2W-2(T) (= MCCC1A02612(T) = LMG 27145(T)).