期刊
JOURNAL OF VIROLOGY
卷 79, 期 20, 页码 13190-13194出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.79.20.13190-13194.2005
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Nonviral producer cell proteins incorporated into retroviral vector surfaces profoundly influence infectivity and in vivo half-life. We report the purification and concentration of lentiviral vectors using these surface proteins as an efficient gene transduction strategy. Biotinylation of these proteins and streptavidin paramagnetic particle concentration enhances titer 400- to 2,500-fold (to 10(9) CFU/ml for vesicular stomatitis virus G protein and 5 x 108 for amphotropic murine leukemia virus envelope). This method also uses newly introduced membrane proteins (B7.1 and Delta LNGFR) directed to lentiviral surfaces, allowing up to 17,000-fold concentrations. Particle conjugation of lentivirus allows facile manipulation in vitro, resulting in the transduction of 48 to 94% of human acute myeloid leukemia blasts.
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