期刊
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
卷 105, 期 3, 页码 501-510出版社
SPRINGER
DOI: 10.1007/s10482-013-0102-3
关键词
Bacillus bingmayongensis; Emperor Qin's Terra-cotta Warriors; Polyphasic taxonomy; Average nucleotide identity
类别
资金
- National fund projects [31370059]
- National Key Project of Fundamental Scientific Research (973 Program) [2011CB111607]
- Agriculture Science & Technology Achievement Transformation [2010GB2C400220]
- International Science & Technology Cooperation [2012DFA31120]
- Agroscientific Research in the Public Interest [201303094]
- Chinese Ministry of Agriculture [2011-G25]
A Bacillus-like isolate, strain FJAT-13831(T), isolated from the No. 1 pit soil of Emperor Qin's Terra-cotta Warriors in Xi'an City, China, was studied to determine its taxonomic status. Dominant fatty acids of this organism included iso-C-15:0, iso-C-17:0, C-16:0, iso-C-13:0, anteiso-C-15:0, and iso-C-17:1 omega 5c. Comparative 16S rRNA gene sequence analysis confirmed the affiliation of this isolate to the genus Bacillus and indicated that it was closely related to Bacillus pseudomycoides DSM 12442(T) (99.72 % similarity). A phylogenetic analysis of the gyrB gene sequence similarities exhibited independent clustering of the isolate FJAT-13831(T) and showed 93.8 % (< 95 %) sequence similarity with its closest phylogenetic neighbour B. pseudomycoides DSM 12442(T). Separate standing of the strain FJAT-13831(T) was supported by a whole genome-based phylogenetic analysis with an average nucleotide identity value of 91.47 (< 95 %) between isolate FJAT-13831(T) and B. pseudomycoides DSM 12442(T) and was consistent with the results of DNA-DNA hybridization (69.1 % relatedness). These findings support the conclusion that the isolate FJAT-13831(T) represents a novel species, for which the name Bacillus bingmayongensis sp. nov. is proposed. The type strain is FJAT-13831(T) (= CGMCC 1.12043(T) = DSM 25427(T)).
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