期刊
PROTEOMICS
卷 5, 期 15, 页码 3831-3835出版社
WILEY
DOI: 10.1002/pmic.200401235
关键词
albumin; 2-DE; enrichment; serum proteome
资金
- NHLBI NIH HHS [N01 HV028180] Funding Source: Medline
The protein constituents of serum can range from grams to picograms per liter, making it tech- Received: July 16, 2004 nically difficult to achieve in-depth proteomic analysis. Removal of highly abundant proteins, Revised: December 29, 2004 such as albumin, coupled to powerful protein separation methods is required for increased Accepted: January 5, 2005 14 sample load, thus facilitating detection and identification of low-abundant proteins. We report here a chemical-based extraction method for the effective and specific removal of albumin from serum.
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