Cohnella plantaginis sp. nov., a novel nitrogen-fixing species isolated from plantain rhizosphere soil

A novel bacterium capable of fixing nitrogen was isolated from plantain rhizosphere soil in China. The isolate, designated YN-83(T), is Gram-positive, aerobic, motile and rod-shaped (0.4-0.6 mu m x 1.9-2.6 mu m). Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain YN-83(T) was a member of the genus . High similarity of 16S rRNA gene sequence was found between YN-83(T) and DSM18997(T) (97.99%), whereas the similarity was below 96.0% between YN-83(T) and the other species. DNA-DNA relatedness between strain YN-83(T) and DSM18997(T) was 27.4 +/- A 6.2%. The DNA G+C content of strain YN-83(T) was 59.3 mol%. The predominant isoprenoid quinone was MK-7 and the major fatty acids were anteiso-C-15:0 (44.3%), iso-C-15:0 (11.3%), iso-C-16:0 (18.6%) and C-16:0 (7.7%). The polar lipids of strain YN-83(T) consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, lyso- phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. On the basis of phenotypic and chemotaxonomic properties, 16S rRNA gene sequence, G+C content and DNA-DNA hybridization, strain YN-83(T) represents a novel species of the genus , for which the name sp. nov. (type strain YN-83(T) = DSM 25424(T) = CGMCC 1.12047(T)) is proposed.