期刊
MOLECULAR MICROBIOLOGY
卷 58, 期 2, 页码 510-519出版社
BLACKWELL PUBLISHING
DOI: 10.1111/j.1365-2958.2005.04838.x
关键词
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资金
- NIAID NIH HHS [R37 AI033493-10, R37 AI033493, R01 AI033493, R01 AI044239-06A2, R01 AI033493-09, F32 AI056681-02, F32 AI056681-01, R01 AI044239, R01 AI044239-05, F32 AI056681] Funding Source: Medline
The pilin antigenic variation (Av) system of Neisseria gonorrhoeae (Gc) mediates unidirectional DNA recombination from silent gene copies into the pilin expression locus. A DNA sequencing assay was developed to accurately measure pilin Av in a population of Gc strain FA1090 arising from a defined pilin progenitor under non-selective culture conditions. This assay employs a piliated parental Gc variant with a recA allele whose promoter is replaced by lac-regulatory elements, allowing for controlled induction of pilin Av. From this assay, the frequency of pilin Av was measured as 0.13 recombination events per cell, with a corresponding rate of pilin Av of 4 x 10(-3) events per cell per generation. Most pilin variants retained the parental piliation phenotype, providing the first comprehensive analysis of piliated variants arising from a piliated progenitor. Sequence analysis of pilin variants revealed that a subset of possible recombination events predominated, which differed between piliated and non-piliated progeny. Pilin Av exhibits the highest reported frequency of any pathogenic gene conversion system and can account for the extensive pilin variation detected during human infection.
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