期刊
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
卷 99, 期 4, 页码 739-751出版社
SPRINGER
DOI: 10.1007/s10482-011-9548-3
关键词
Miscellaneous crenarchaeotal group; Methyl coenzyme-M reductase; Pearl River estuarine sediments
类别
资金
- National Basic Research Program of China [2011CB808800]
- Natural Science Foundation of China [40830213]
- National High-Tech Program [2007AA 091904]
- COMRA [DYXM-115-02-2-01]
- Third Institute of Oceanography, State Oceanic Administration
- State Key Laboratory of Marine Geology, Tongji University
Microorganisms are known to play fundamental roles in the biogeochemical cycling of carbon in the coastal environments. To get to know the composition and ecological roles of the archaeal communities within the sediments of the Pearl River Estuary, Southern China, the diversity and vertical distribution of archaea in a sediment core was reported based on the 16S rRNA and mcrA genes for the first time. Quantitative PCR analysis revealed that archaea were present at 10(6)-10(7) 16S rRNA gene copies/g (wet weight) in the sediment core, and the proportion of mcrA versus 16S rRNA gene copies varied from 11 to 45%. 16S rRNA gene libraries were constructed and analyzed for the top layer (0-6 cm), middle layer (18-24 cm), sulfate-methane transition zone (SMTZ, 32-42 cm), and bottom layer (44-50 cm) sediments. The results indicated that Miscellaneous Crenarchaeotal Group (MCG) was the main component in the sediments. The MCG archaea could be further divided into six subgroups: MCG-A, B, C, D, E, and F. On the other hand, mcrA sequences from methanogens related to the order Methanomicrobiales and ANME-2 methanotrophs were detected in all sediment layers. Taken together, our data revealed a largely unknown archaeal community in which MCG dominated within the Pearl River estuarine sediments, while methanogens and methane-oxidizing archaea putatively involving in methane metabolism, were also found in the community. This is the first important step towards elucidating the biogeochemical roles of these archaea in the Pearl River Estuary.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据