期刊
JOURNAL OF THE SOCIETY FOR GYNECOLOGIC INVESTIGATION
卷 12, 期 7, 页码 504-509出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jsgi.2005.06.003
关键词
amniotic fluid volume; fetal swallowing; glutamate-NMDA receptors
资金
- NICHD NIH HHS [K08 HD 40251-02] Funding Source: Medline
- NIDDK NIH HHS [R01 DK43311] Funding Source: Medline
BACKGROUND: In adult rats, N-methyl-D-aspartate (NMDA) receptors have been implicated in the central control of body fluid homeostasis, as intracerebroventricular (ICV) infection of NMDA receptor antagonists suppresses stimulated drinking behavior. Fetal swallowing occurs at a significantly higher rate as compared to adult drinking, contributing to amniotic fluid volume regulation and fetal gastrointestinal development. The aim of present study was to determine the role of central NMDA receptors in the modulation of fetal swallowing activity. METHODS: Eight time-dated pregnant ewes and fetuses were chronically prepared with fetal vascular and ICV catheters, electrocorticogram (ECoG), and esophageal electromyogram electrodes and studied at 130 +/- 1 days' gestation. Following an initial 2-hour baseline period (the 2h), the NMDA receptor antagonist, dizocipline (1 mg), was infected ICV At time 4h, the dose of dizocipline was repeated, together with angiotensin II (AngII, 6.4 mu g). Fetal swa llowing, was monitored for 2 hours after each injection. Four of these fetuses also received an identical control study, (on an alternate day) in which dizocipline was replaced with artificial cerebrospinal fluid (aCSF). RESULTS: ICV dizocipline injection nearly abolished spontaneous fetal swallowing activities (0.6 - 0.1 to 0.2 +/- 0.1 swalloms/min; P <. 001). ICV Ang II in the presence of dizocipline did not demonstrate a dipsogenic affect on fetal swallowing (0.1 +/- 0.1 P <. 001). In the control study, ICV injection of aCSF did not change fetal swallowing activity (1.0 +/- 0.1 swallows/min), whil ICV AngII resulted in a significant increase in fetal swallowing (2.0 +/- 0.1 swallows/min; P <.001). CONCLUSIONS: This study demonstrates that central NMDA-glutamate receptor-mediated activity contributes to the high rate of spontaneous and AngII-stimulated fetal swallowing. We speculate that reduced NMDA receptor expression within the forebrain dipsogenic neurons may account for observed differences in drinking activities between the fetus/neonate and the adult.
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