期刊
EMBO REPORTS
卷 6, 期 10, 页码 961-967出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.embor.7400509
关键词
Dicer; TRBP; PKR; RNA interference; microRNA
Dicer is a key enzyme involved in RNA interference (RNAi) and microRNA ( miRNA) pathways. It is required for biogenesis of miRNAs and small interfering RNAs (siRNAs), and also has a role in the effector steps of RNA silencing. Apart from Argonautes, no proteins are known to associate with Dicer in mammalian cells. In this work, we describe the identification of TRBP ( human immunodeficiency virus (HIV-1) transactivating response ( TAR) RNA-binding protein) as a protein partner of human Dicer. We show that TRBP is required for optimal RNA silencing mediated by siRNAs and endogenous miRNAs, and that it facilitates cleavage of pre-miRNA in vitro. TRBP had previously been assigned several functions, including inhibition of the interferon-induced double-stranded RNA-regulated protein kinase PKR and modulation of HIV-1 gene expression by association with TAR. The TRBP - Dicer interaction shown raises interesting questions about the potential interplay between RNAi and interferon - PKR pathways.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据