期刊
ANTIVIRAL RESEARCH
卷 104, 期 -, 页码 128-135出版社
ELSEVIER
DOI: 10.1016/j.antiviral.2014.01.020
关键词
Classical swine fever virus (CSFV); Porcine Mx1 (poMx1); Antiviral activity
资金
- National Natural Science Foundation of China [31001062]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
Classical swine fever virus (CSFV) is the causative pathogen of classical swine fever (CSF), a highly contagious disease of swine. Mx proteins are interferon-induced dynamin-like GTPases present in all vertebrates with a wide range of antiviral activities. Although Zhao et al. (2011) have reported that human MxA can inhibit CSFV replication, whether porcine Mxl (poMx1) has anti-CSFV activity remains unknown. In this study, we generated a cell line designated PK-15/EGFP-poMx1 which expressed porcine Mxl protein constitutively, and we observed that the proliferation of progeny virus in this cell line was significantly inhibited as measured by virus titration, indirect immune fluorescence assay, Q-PCR and Western blot. Furthermore, when PTD-poMx1 fusion protein expressed in Escherichia coli (Zhang et al., 2013) was used to treat CSFV-infected PK-15 cells, the results showed that PTD-poMx1 inhibited CSFV replication in a dose-dependent manner. Additionally, the proliferation of progeny virus was inhibited as measured by virus titration and Q-PCR. Overall, the results demonstrated that poMx1 effectively inhibited CSFV replication, suggesting that poMx1 may be a valuable therapeutic agent against CSFV infection. (C) 2014 Elsevier B.V. All rights reserved.
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