期刊
ANTIVIRAL RESEARCH
卷 105, 期 -, 页码 126-134出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.antiviral.2014.02.007
关键词
Ethnomedicine; Ophiorrhiza nicobarica; HSV; Immediate-early transcription; LSD1
资金
- Department of Biotechnology, Govt. of India
- Indian Council of Medical Research, Govt. of India
In our continued quest for identifying novel molecules from ethnomedicinal source we have isolated an alkaloid 7-methoxy-1-methyl-4,9-dihydro-3H-pyrido[3,4-b]indole, also known as Harmaline (HM), from an ethnomedicinal herb Ophiorrhiza nicobarica. The compound exhibited a potent anti-HSV-1 activity against both wild type and clinical isolates of HSV-1. Further we demonstrated that HM did not interfere in viral entry but the recruitment of lysine-specific demethylase-1 (LSDI) and the binding of immediate-early (IE) complex on ICPO promoter. This leads to the suppression of viral IE gene synthesis and thereby the reduced expression of ICP4 and ICP27. Moreover, HM at its virucidal concentration is nontoxic and reduced virus yields in cutaneously infected Balb/C mice. Thus, the interference in the binding of IE complex, a decisive factor for HSV lytic cycle or latency by HM reveals an interesting target for developing non-nucleotide antiherpetic agent with different mode of action than Acyclovir. (C) 2014 Elsevier B.V. All rights reserved.
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