期刊
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY
卷 89, 期 2, 页码 124-152出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.pbiomolbio.2004.09.003
关键词
synchrotron; macromolecular crystallography; beamline; automation
The production of three-dimensional crystallographic structural information of macromolecules can now be thought of as a pipeline which is being streamlined at every stage from protein cloning, expression and purification, through crystallisation to data collection and structure solution. Synchrotron X-ray beamlines are a key section of this pipeline as it is at these that the X-ray diffraction data that ultimately leads to the elucidation of macromolecular structures are collected. The burgeoning number of macromolecular crystallography (MX) beamlines available worldwide may be enhanced significantly with the automation of both their operation and of the experiments carried out on them. This paper reviews the current situation and provides a glimpse of how a NIX beamline may look in the not too distant future. (c) 2004 Elsevier Ltd. All rights reserved.
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