期刊
ANTIVIRAL RESEARCH
卷 101, 期 -, 页码 122-130出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.antiviral.2013.11.006
关键词
Coronavirus; SARS-CoV; Replication; Transcription; Nonstructural proteins
资金
- French National Research agency [ANR-08-MIEN-032, ANR-12-BSV3]
- Fondation pour la Recherche Medicale (Programme Equipe FRM)
- European Union Seventh Framework Programme through the project SILVER (Small Inhibitor Leads Versus Emerging and Negleted RNA viruses) [260644]
- Infectiopole Sud
- Direction Generale pour l'Armement [07co404]
- Marie Curie Fellowship through the EUVIRNA project
The SARS (severe acute respiratory syndrome) pandemic caused ten years ago by the SARS-coronavirus (SARS-CoV) has stimulated a number of studies on the molecular biology of coronaviruses. This research has provided significant new insight into many mechanisms used by the coronavirus replication-transcription complex (RTC). The RTC directs and coordinates processes in order to replicate and transcribe the coronavirus genome, a single-stranded, positive-sense RNA of outstanding length (similar to 27-32 kilobases). Here, we review the up-to-date knowledge on SARS-CoV replicative enzymes encoded in the ORF1b, i.e., the main RNA-dependent RNA polymerase (nsp12), the helicase/triphosphatase (nsp13), two unusual ribonucleases (nsp14, nsp15) and RNA-cap methyltransferases (nsp14, nsp16). We also review how these enzymes co-operate with other viral co-factors (nsp7, nsp8, and nsp10) to regulate their activity. These last ten years of research on SARS-CoV have considerably contributed to unravel structural and functional details of one of the most fascinating replication/transcription machineries of the RNA virus world. This paper forms part of a series of invited articles in Antiviral Research on From SARS to MERS: 10 years of research on highly pathogenic human coronaviruses. (C) 2013 Published by Elsevier B.V.
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