期刊
ANTIVIRAL RESEARCH
卷 98, 期 2, 页码 171-173出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.antiviral.2013.02.015
关键词
Antivirus; BmNPV; RNAi; Overexpression; Silkworm; Transgenic
资金
- National Basic Research Program of China [2012CB114600]
- National Hi-Tech Research and Development Program of China [2011AA100306, 2013AA102507]
- Foundation for Sci & Tech Research Project of Chongqing [CSTC2009AA1024]
- National Natural Science Foundation of China [30901054]
- Production, Education & Research of Guangdong
- Ministry of Education [2010A090200079]
Bombyx mori nucleopolyhedrovirus (BmNPV) is the primary pathogen of silkworms, causing severe economic losses in sericulture. To create antiviral silkworm strains, we constructed a transgenic vector in which the dsRNA for five tandem BmNPV genes was controlled by the BmNPV hr3 enhancer and IE1 promoter. The antivirus gene Bmlipase-1 was driven by B. mori midgut-specific promoter P2. Transgenic strains (SW-H) were generated via embryo microinjection using the practical silkworm strain SW. After infection with a high dose of BmNPV, the survival rates of SW-H and non-transgenic SW were 64% and 13%, respectively. SW-H could be the first transgenic animal that is highly antiviral and that might inhibit the virus at multiple stages of infection. (C) 2013 Elsevier B.V. All rights reserved.
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