期刊
ANTIVIRAL RESEARCH
卷 100, 期 2, 页码 460-472出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.antiviral.2013.08.002
关键词
Influenza virus; (-)-Epigallocatechin-3-gallate; Antiviral activity; Viral entry; Membrane damage
资金
- Transgovernmental Enterprise for Pandemic Influenza in Korea (TEPIK) [A103001]
(-)-Epigallocatechin-3-gallate (EGCG), one of the major flavonoid components of green tea, is known to have a broad antiviral activity against several enveloped viruses, including the influenza virus. However, its mode of action and the mechanism that allows it to target influenza virus molecules have not been fully elucidated. Thus, this study investigated the molecular mechanism by which EGCG suppresses influenza virus infections. EGCG was found to block an early step in the influenza viral life cycle, but it did not affect viral adsorption to target cells or viral RNA replication. However, EGCG inhibited hemifusion events between virus particles and the cellular membrane by reducing the viral membrane integrity, thereby resulting in the loss of the cell penetration capacity of the influenza virus. EGCG also marginally suppressed the viral and nonviral neuraminidase (NA) activity in an enzyme-based assay system. In conclusion, it is suggested that the anti-influenza viral efficacy of EGCG is attributable to damage to the physical properties of the viral envelope and partial inhibition of the NA surface glycoprotein. These results may facilitate future investigations of the antiviral activity of EGCG against other enveloped viruses as well as influenza virus. (C) 2013 Elsevier B.V. All rights reserved.
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