期刊
ANTIVIRAL RESEARCH
卷 92, 期 2, 页码 237-246出版社
ELSEVIER
DOI: 10.1016/j.antiviral.2011.08.010
关键词
Influenza A virus; Carrageenan oligosaccharide; Adsorption; Internalization; Cell surface
资金
- Program for Changjiang Scholars and Innovative Research Team in University [IRT0944]
- Fundamental Research Funds for the Central Universities [201113013]
- Shandong Provincial Natural Science Foundation [ZR2011HQ012]
- Special Fund for Marine Scientific Research in the Public Interest [201005024]
- National Basic Research Program of China [2006CB933102]
Carrageenan polysaccharide has been reported to be able to inhibit the infection and replication of many different kinds of viruses. Here, we demonstrated that a 2 kDa kappa-carrageenan oligosaccharide (CO-1) derived from the carrageenan polysaccharide, effectively inhibited influenza A (H1N1) virus replication in MDCK cells (selectivity index >25.0). Moreover, the 2 kDa CO-1 inhibited influenza A virus (IAV) replication better than that of 3 kDa and 5 kDa kappa-carrageenan oligosaccharides (CO-2 and CO-3). IAV multiplication was suppressed by carrageenan oligosaccharide treatment in a dose-dependent manner. Carrageenan oligosaccharide CO-1 did not bind to the cell surface of MDCK cells but inactivated virus particles after pretreatment. Different to the actions of carrageenan polysaccharide, CO-1 could enter into MDCK cells and did not interfere with IAV adsorption. CO-1 also inhibited IAV mRNA and protein expression after its internalization into cells. Moreover, carrageenan oligosaccharide CO-1 had an antiviral effect on IAV replication subsequent to viral internalization but prior to virus release in one replication cycle. Therefore, inhibition of IAV intracellular replication by carrageenan oligosaccharide might be an alternative approach for anti-influenza A virus therapy. (C) 2011 Elsevier B.V. All rights reserved.
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