期刊
JOURNAL OF BACTERIOLOGY
卷 187, 期 20, 页码 6874-6882出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.187.20.6874-6882.2005
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资金
- NHGRI NIH HHS [1 P20 HG003638-01, P20 HG003638] Funding Source: Medline
- NIGMS NIH HHS [F32 GM067472] Funding Source: Medline
- PHS HHS [F32 G067472] Funding Source: Medline
Cryoelectron microscope tomography (cryoEM) and a fluorescence loss in photobleaching (FLIP) assay were used to characterize progression of the terminal stages of Caulobacter crescentus cell division. Tomographic cryoEM images of the cell division site show separate constrictive processes closing first the inner membrane (IM) and then the outer membrane (OM) in a manner distinctly different from that of septum-forming bacteria. FLIP experiments had previously shown cytoplasmic compartmentalization (when cytoplasmic proteins can no longer diffuse between the two nascent progeny cell compartments) occurring 18 min before daughter cell separation in a 135-min cell cycle so the two constrictive processes are separated in both time and space. In the very latest stages of both IM and OM constriction, short membrane tether structures are observed. The smallest observed prefission tethers were 60 nm in diameter for both the inner and outer membranes. Here, we also used FLIP experiments to show that both membrane-bound and periplasmic fluorescent proteins diffuse freely through the FtsZ ring during most of the constriction procession.
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