期刊
ACTA OPHTHALMOLOGICA SCANDINAVICA
卷 83, 期 5, 页码 543-548出版社
WILEY
DOI: 10.1111/j.1600-0420.2005.00482.x
关键词
alpha-smooth muscle actin; keratocytes; wound healing; ultraviolet radiation
Purpose: This study was designed to demonstrate the expression of the chaperonin containing T-complex polypeptide 1 (CCT) and alpha-smooth muscle actin (alpha-SMA), in normal corneas and corneas treated with ultraviolet radiation (UVR). The wound model chosen is previously characterized, the injury is mild and the cornea heals to transparency. Methods: Rabbit corneas were exposed to UVR at the dose producing keratitis. The corneas were allowed to heal for up to 5 days and the paraffin-embedded tissue specimens were double stained and examined morphologically and immunohistochemically. Expression of CCT and alpha-SMA genes was investigated by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). Results: There was a front of repopulating keratocytes that showed positive staining for alpha-SMA after 3 days. The alpha-SMA mRNA was already strongly expressed after 1 day, whereas the expression level of CCT was increased after 2 days. After 5 days the levels were decreased. By this time the stroma was partly repopulated by keratocytes. Conclusion: In a mild wound, the expression of alpha-SMA mRNA is followed by expression of mRNA of at least one subunit of the complex folding alpha-SMA. At protein level, alpha-SMA is detected in the front line of repopulating keratocytes. Expression levels for both mRNAs decline as the stroma repopulation process progresses.
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