期刊
ANALYTICAL CHEMISTRY
卷 77, 期 19, 页码 6229-6233出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac050921y
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资金
- NIA NIH HHS [AG18231] Funding Source: Medline
We have used the disparity in adsorption rates for single- and double-stranded RNA on ionically coated gold nanoparticles suspended in a colloid to design a rapid sequence identification assay. Unlabeled target RNA and a probe sequence are mixed prior to exposure to the gold nanoparticles to enable efficient hybridization. We have designed assays based on either color changes or fluorescence that are sensitive to a few picomoles of target. Single-base mutations on RNA sequences can be detected even in complex oligonucleotide mixtures. The assay requires less than 10 min so that RNA degradation problems are avoided.
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