期刊
BIOPHYSICAL JOURNAL
卷 89, 期 4, 页码 2458-2472出版社
CELL PRESS
DOI: 10.1529/biophysj.105.062539
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- NIGMS NIH HHS [R01 GM056827, GM 56827] Funding Source: Medline
- NIMH NIH HHS [R01 MH061876, MH61876] Funding Source: Medline
Docking and fusion of single proteoliposomes reconstituted with full-length v-SNAREs ( synaptobrevin) into planar lipid bilayers containing binary t-SNAREs ( anchored syntaxin associated with SNAP25) was observed in real time by wide-field fluorescence microscopy. This enabled separate measurement of the docking rate k(dock) and the unimolecular fusion rate k(fus). On low t-SNARE-density bilayers at 37 degrees C, docking is efficient: k(dock) = 2.2 x 3 10(7) M-1 s(-1), similar to 40% of the estimated diffusion limited rate. Full vesicle fusion is observed as a prompt increase in fluorescence intensity from labeled lipids, immediately followed by outward radial diffusion ( D-lipid = 0.6 mu m(2) s(-1)); similar to 80% of the docked vesicles fuse promptly as a homogeneous subpopulation with k(fus) = 40 +/- 15 s(-1) (tau(fus) = 25 ms). This is 10(3) - 10(4) times faster than previous in vitro fusion assays. Complete lipid mixing occurs in, 15 ms. Both the v-SNARE and the t-SNARE are necessary for efficient docking and fast fusion, but Ca2+ is not. Docking and fusion were quantitatively similar on syntaxin-only bilayers lacking SNAP25. At present, in vitro fusion driven by SNARE complexes alone remains; 40 times slower than the fastest, submillisecond presynaptic vesicle population response.
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