期刊
ANTIVIRAL RESEARCH
卷 79, 期 2, 页码 114-120出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.antiviral.2008.03.001
关键词
Geranium carolinianum L.; polyphenolic extract; hepatitis B virus (HBV); duck hepatitis B virus (DHBV)
Natural products provide a large reservoir of potentially active agents with anti-hepatitis B virus (HBV) activity. We examined the effect of the polyphenolic extract from Geranium carolinianum L. (PPGC) on HBV replication both in vitro and in vivo. In the human HBV-transfected liver cell line HepG(2) 2.2.15, PPGC effectively suppressed the secretion of the HBV antigens in a dose-dependent manner with IC50 values of 46.85 mu g/ml for HBsAg and 65.60 mu g/ml for HBeAg at day 9. Consistent with the HBV antigen reduction, PPGC (100 mu g/ml) also reduced HBV DNA level by 35.9%. In the duck hepatitis B virus (DHBV) infected ducks, after PPGC was dosed intragastricly (i.g.) once a day for 10 days, the plasma DHBV DNA level was reduced, with an ED50 value of 47.54mg/kg. In addition, Southern blot analysis confirmed the in vivo anti-HBV effect of PPGC in ducks and PPGC also reduced the plasma and the liver DHBV DNA level in a dose-dependent manner. Furthermore, significant improvement of the liver was observed after PPGC treatment, as evaluated by the histopathological analysis. (C) 2008 Elsevier B.V. All rights reserved.
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