期刊
FEMS MICROBIOLOGY ECOLOGY
卷 54, 期 2, 页码 247-255出版社
OXFORD UNIV PRESS
DOI: 10.1016/j.femsec.2005.03.010
关键词
symbiosis; 16S rRNA gene; clone library; fluorescence in situ hybridization; Actinobacteria
类别
The bacterial community colonizing the gut wall of the termite Reticulitermes speratus was characterized without cultivation. Analysis of 16S rRNA genes after fractionation of the gut revealed that the bacterial composition on the gut wall was diverse and significantly different from that able to move unconfined in the gut fluid or physically associated with the gut protists. Actinobacteria, Firmicutes and Baeteroidetes were dominant on the gut wall, but Spiroehaetes and the Termite group 1 phylum, abundant in the gut lumen, were relatively rare. A sequence-specific probe enabled the in situ detection of a rod-shaped Actinobacteria member, abundantly colonizing the gut paunch epithelium. (C) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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