Characterization of a novel bioactive poly[(lactic acid)-co-(glycolic acid)] and collagen hybrid matrix for dermal regeneration

Different strategies have been explored for the purpose of autologous or allogeneic dermal regeneration. We have developed a hybrid matrix by lyophilizing collagen within a poly[(lactic acid)co(glycolic acid)] (10:90, molar composition) knitted mesh, in order to assimilate the advantages of natural and synthetic materials. The porosity of the mesh was found to be almost 95 %, using Micro-Computed Tomography Analysis, while the mechanical properties were comparable to native skin. In vitro biocompatibility was analyzed by culturing rat dermal fibroblasts in the matrices over 10 days. The cells were able to attach, proliferate and remain viable within the hybrid matrices. Subsequently, in vivo biocompatibility was analyzed by implanting the matrices subcutaneously in immunocompetent rats, for 2 weeks. Histological analysis showed that the poly[(lactic acid)co (glycolic acid)]-collagen hybrid matrices evoked minimal host tissue response in vivo. This study forms the basis of using poly[(lactic acid)co(glycolic acid)]-collagen hybrid matrices for our future work to develop a bioactive matrix for dermal regeneration. (c) 2005 Society of Chemical Industry.