期刊
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
卷 289, 期 4, 页码 H1635-H1642出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpheart.00016.2005
关键词
BK channel beta 1-subunit; beta 1-subunit; cytochrome c oxidase; cardioprotection
The molecular components of the large-conductance Ca2+-activated K+ channels that are functionally expressed in mitochondria (mitoK(Ca)) in cardiac myocytes have not been identified. Our experimental results show that the transcript corresponding to the large-conductance Ca2+-activated K+ channel beta 1-subunit (BK-beta 1) is substantially expressed in mammalian heart. A yeast two-hybrid assay showed the BK-beta 1 protein can interact with a mitochondrial protein, cytochrome c oxidase subunit I (Cco1). Results from immunocytochemical experiments also demonstrated that BK-beta 1 interacted with Cco1 and colocalized in rat cardiac mitochondria. Furthermore, 17 beta-estradiol, which enhances the activity of the BK channel alpha-subunit only in the presence of the beta 1-subunit, significantly increased flavoprotein oxidation in rat ventricle myocytes and decreased the rate of cell death under simulated ischemia. Single-channel recordings from mitochondrial inner membrane indicated that the activity of mitoK(Ca), which had a conductance of similar to 270 pS, was enhanced by 17 beta-estradiol and blocked by paxilline. In combination, the present study revealed a new mechanism for the cardioprotective effects of 17 beta-estradiol, which include the activation of mitoK(Ca) via the interaction with BK-beta 1. BK-beta 1 may be an important molecular component that functionally couples with both Cco1 and mitoK(Ca) pore-forming alpha-subunit.
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