期刊
PEPTIDES
卷 26, 期 10, 页码 1842-1847出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.peptides.2004.11.037
关键词
ACTH; human melanocortin-2 receptor; stable expression; enhanced green fluorescent protein
The human melanocortin-2 receptor (hMC2R) is mainly present in the adrenal cortex and has been difficult to express in heterologous cells. The hMC2R fused to the EGFP at its C-terminus has been stably transfected in the murine M3 melanoma and HEK293 cells. In the M3 cells, the hMC2R-EGFP was well-addressed to the cell membrane and functional whereas in the HEK293 cells, the hMC2R-EGFP was retained intracellularly. These results suggest that some specific factors, missing in cells, which do not express any melanocortin receptor, are involved in the correct addressing of the hMC2R to the cell membrane. (c) 2005 Elsevier Inc. All rights reserved.
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