期刊
JOURNAL OF BACTERIOLOGY
卷 187, 期 20, 页码 7027-7037出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.187.20.7027-7037.2005
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资金
- NCI NIH HHS [5-R01-CA86061, R01 CA086061, R01 CA080024, 1-R01-CA-80024] Funding Source: Medline
- NIEHS NIH HHS [1-P01-ES-11399, U19 ES011399] Funding Source: Medline
- NIGMS NIH HHS [R01 GM063790-03, R01 GM063790, GM63790, T32 GM008334, 2-T32-GM08334] Funding Source: Medline
DNA adenine methylation by DNA adenine methyltransferase (Dam) in Escherichia coli plays an important role in processes such as DNA replication initiation, gene expression regulation, and mismatch repair. In addition, E. coli strains deficient in Dam are hypersensitive to DNA-damaging agents. We used genome microarrays to compare the transcriptional profiles of E. coli strains deficient in Dam and mismatch repair (dam, dam mutS, and mutS mutants). Our results show that > 200 genes are expressed at a higher level in the dam strain, while an additional mutation in mutS suppresses the induction of many of the same genes. We also show by microarray and semiquantitative real-time reverse transcription-PCR that both dam and dam mutS strains show derepression of LexA-regulated SOS genes as well as the up-regulation of other non-SOS genes involved in DNA repair. To correlate the level of SOS induction and the up-regulation of genes involved in recombinational repair with the level of DNA damage, we used neutral single-cell electrophoresis to determine the number of double-strand breaks per cell in each of the strains. We find that dam mutant E. coli strains have a significantly higher level of double-strand breaks than the other strains. We also observe a broad range in the number of double-strand breaks in dam mutant cells, with a minority of cells showing as many as 10 or more double-strand breaks. We propose that the up-regulation of recombinational repair in dam mutants allows for the efficient repair of double-strand breaks whose formation is dependent on functional mismatch repair.
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